INTRACELLULAR SYNTHESIS OF CHONDROITIN SULFATE
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چکیده
منابع مشابه
Intracellular Synthesis of Chondroitin Sulfate
In autoradiograms of slices of costal cartilage, incubated for 4 hours in a salt solution containing S(35)-sulfate and then washed extensively and dehydrated, about 85 per cent of the radioactivity was assignable to the chondrocytes. From alkaline extracts of similarly prepared slices of cartilage, 64 to 83 per cent of the total sulfur-35 in the slices was isolated as chondroitin sulfate by chr...
متن کاملThe Occurrence of Intracellular Chondroitin Sulfate
Suspensions of chondrocytes were prepared by treatment with trypsin of the epiphyses of tibias and femurs of 13-day-old chick embryos. After washing to remove the matrix, such suspensions readily incorporate radioactive sulfate into both intracellular and extracellular chondroitin sulfate. Following disruption of the cells, the cell constituents were fractionated by centrifugation. Fractions ob...
متن کاملRegulation of Chondroitin Sulfate Synthesis EFFECT OF P-XYLOSIDES ON SYNTHESIS OF CHONDROITIN SULFATE PROTEOGLYCAN, CHONDROITIN SULFATE CHAINS, AND CORE PROTEIN*
Monolayer cultures of embryonic chick chondrocytes were incubated with %04*in the presence and absence of 1.0 mM p-nitrophenyl-/3-o-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of P-xyloside-initiated polysaccharide chains was accompanied by an apparent decrease in...
متن کاملRegulation of chondroitin sulfate synthesis. Effect of beta-xylosides on synthesis of chondroitin sulfate proteoglycan, chondroitin sulfate chains, and core protein.
Monolayer cultures of embryonic chick chondrocytes were incubated with 35SO42- in the presence and absence of 1.0 mM p-nitrophenyl-beta-d-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of beta-xyloside-initiated polysaccharide chains was accompanied by an apparent de...
متن کاملChondroitin sulfate
While some complementary and alternative techniques have been studied scientifically, high-quality data regarding safety, effectiveness, and mechanism of action are limited or controversial for most therapies. Whenever possible, it is recommended that practitioners be licensed by a recognized professional organization that adheres to clearly published standards. In addition, before starting a n...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1962
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.13.3.359